Monitoring Growth of Beer Brewing Strains of Saccharomyces Cerevisiae The Utility of SynergyTM H1 for Providing High Quality Kinetic Data for Yeast Growth Applications

Strains of the yeast Saccharomyces cerevisiae serve critical roles in the production of many food staples such as beer, bread and wine. The fermentation of beer or wine yeast converts complex mixtures of sugars, amino acids, peptides, proteins, nucleic acids and numerous other compounds into alcohol, carbon dioxide and the desired fl avors of the intended beverage. The ability to obtain the desired product reliably and repeatedly requires careful monitoring of not only the input materials, but the growth of the yeast strain during the process. Likewise the development of new strains often requires monitoring the growth of strains under various conditions. Here we describe the use of the SynergyTM H1 Hybrid Multi-Mode Microplate Reader to provide temperature control, solution agitation, and monitor cellular yeast growth using light scatter in 96-well microplates. Monitoring of Cell Suspensions by Kinetic Absorbance Measurements